siRNA / miRNA gene silencing Human - LNCap MID1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 1 matching solution for this experiment

Dharmacon

Nanofectin

ExCell Bio

Upstream tips	Protocol tips	Downstream tips
	Final concentration of MID1-20 to 40 nM. MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′ Add diluted siRNA to diluted NanoFectin reagent. Incubate for 15 min at RT and add to cells.  Replace medium after 48hand re-incubate cells for 72-120h at 37°C.

Protocol tips
Final concentration of MID1-20 to 40 nM. MID1-3 siRNA: 5′-GUGUGAUACUAGGAUGCGG-3′ Add diluted siRNA to diluted NanoFectin reagent. Incubate for 15 min at RT and add to cells.  Replace medium after 48hand re-incubate cells for 72-120h at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

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