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Thermo Fisher Scientific
|Seed 2.5 × 10^5|
|Daxx siRNA: 5′-GGAGUUGGAUCUCUCAGAA
Add diluted siRNA to diluted Lipofectamine® RNAiMAX Reagent (1:1 ratio).
Incubate for 5 minutes at room temperature and add to cells.
Incubate cells for 1day at 37°C and later transfect by electroporation with 2 μg of circularized wt HPV18 or wt HPV11 genome and incubate for another 48 h
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