siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

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Found 2 matching solutions for this experiment

Nrf2 siRNA (m)

Santa Cruz Biotechnology

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′ siRNA concentration- 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h. Incubate cells for another 24h at 37°C and quantify.

Protocol tips
NRF2-siRNA, 5′-UGAAAGCACAGCAGAAUUTT-3′ and control-siRNA, 5′-GAGCGGCCGAGCAACGUCUAU-3′ siRNA concentration- 50 nM Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h. Incubate cells for another 24h at 37°C and quantify.

Manufacturer protocol Publication protocol 1 Relevant paper

NFE2L2 siRNA

Thermo Fisher Scientific

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	siRNA sequences: 5′-UGGAGCAAGACUUGGGCCACUUAAA-3′ and 5′ UUUAAGUGGCCCAAGUCUUGCUCCA-3′ Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 12h at 37°C.

Protocol tips
siRNA sequences: 5′-UGGAGCAAGACUUGGGCCACUUAAA-3′ and 5′ UUUAAGUGGCCCAAGUCUUGCUCCA-3′ Add diluted siRNA to diluted Lipofectamine Reagent Incubate for 5 min at RT and add to cells. Incubate cells for 12h at 37°C.

Manufacturer protocol Publication protocol 1 Relevant paper

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