siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 2 matching solutions for this experiment

Nrf2 siRNA (m) + Lipofectamine® RNAiMAX Transfection Reagent

Nrf2 siRNA (m)

Santa Cruz Biotechnology

Protocol tips

siRNA concentration- 50 nM

Incubate Lipofectamine reagent:siRNA mixture for 5 min and add to cells for 6h.

Incubate cells for another 24h at 37°C and quantify.

NFE2L2 siRNA + Lipofectamine® 2000 Transfection Reagent


Thermo Fisher Scientific

Protocol tips

Add diluted siRNA to diluted Lipofectamine Reagent

Incubate for 5 min at RT and add to cells.

Incubate cells for 12h at 37°C.
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms