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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
|
Always add your plasmid to the buffer before adding Xfect Polymer.
dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl.
Incubate for 10 min at room temperature.
Add the nanoparticle complex solution dropwise to the cell culture medium.
Incubate the plate at 37°C for 4 hr to overnight. |
|
| Protocol tips |
Always add your plasmid to the buffer before adding Xfect Polymer.
dilute 5 µg of your plasmid DNA with Xfect Reaction Buffer to a final volume of 100 µl.
Incubate for 10 min at room temperature.
Add the nanoparticle complex solution dropwise to the cell culture medium.
Incubate the plate at 37°C for 4 hr to overnight. |
| Upstream tips |
Protocol tips |
Downstream tips |
Seed ~5 × 10^4 cells
|
Dilute 1µg of DNA in 100µL of serum-free DMEM or other serum-free growth medium.
Incubate 15-20 minutes at room temperature.
Add transfection reagent/DNA mixture to samples and incubate at 37°C in a CO2 incubator. |
Analyse expression after 24-48 hours |
| Upstream tips |
Seed ~5 × 10^4 cells
|
| Protocol tips |
Dilute 1µg of DNA in 100µL of serum-free DMEM or other serum-free growth medium.
Incubate 15-20 minutes at room temperature.
Add transfection reagent/DNA mixture to samples and incubate at 37°C in a CO2 incubator. |
| Downstream tips |
| Analyse expression after 24-48 hours |
| Upstream tips |
Protocol tips |
Downstream tips |
|
Dilute siRNA in culture medium without serum.
Incubate the samples for 5–10 min at room temperature |
|
| Protocol tips |
Dilute siRNA in culture medium without serum.
Incubate the samples for 5–10 min at room temperature |
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