Found 1 discussion for this experiment
2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 2 matching solutions for this experiment
|Fix cell samples with Fixation solution for 1 h.
Incubate in Permeabilisation solution (0.1% Triton X-100 in PBS) for 2 min on ice.
Label and incubate for 60 min at +37°C in a humidified atmosphere in the dark
|If low labelling is found, reduce fixation time or try 2% buffered paraformaldehyde|