dna-quantification-human-pc-3

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Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Products R&D Systems Human/Mouse Active Caspase-3 Antibody

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2

Products Biotium Apoptosis and Necrosis Quantification Kit

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Immortalized cell lines OVCAR-3

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Immortalized cell lines SKOV-3

Get tips on using JNK1 Antibody (F-3) to perform Autophagy assay cell type - PC-12

Products Santa Cruz Biotechnology JNK1 Antibody (F-3)
siRNA SKIL Product

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - PC-3 SKIL

Products Thermo Fisher Scientific siRNA SKIL

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3 uPAR

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - PC-12

Products Thermo Fisher Scientific Pierce™ BCA Protein Assay Kit

Get tips on using Human Chitinase 3-like 1 DuoSet ELISA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Products R&D Systems Human Chitinase 3-like 1 DuoSet ELISA

Get tips on using PACE4 siRNA (h) to perform siRNA / miRNA gene silencing Human - PC-3 PACE4

Products Santa Cruz Biotechnology PACE4 siRNA (h)

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