pcr-multiplex-pcr-bacterial-dna

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using QuantiTect Multiplex PCR NoROX Kit (1000) to perform PCR Multiplex PCR - Poultry DNA

Get tips on using Phusion U Multiplex PCR Master Mix to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using QuantiNova Multiplex RT-PCR Kit (2500) to perform PCR Multiplex PCR - Viral

Get tips on using QuantiTect Multiplex RT-PCR Kit (1000) to perform PCR Multiplex PCR - Viral

Get tips on using QuantiTect Multiplex RT-PCR NR Kit (1000) to perform PCR Multiplex PCR - Viral

I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?

Get tips on using PerfeCTa MultiPlex qPCR SuperMix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Promega PCR Master Mix to perform PCR Multiplex PCR - Bacterial DNA