wound-healing-assay-cell-type-human-a375

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse 4T1

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse NIH 3T3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Cell migration / Invasion cell type - HaCat

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human A375

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human BEAS2B

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human A549

Get tips on using Oris™ Cell Migration Assay to perform Wound healing assay cell type - human MCF-10A

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human HUVEC