rna-isolation-purification-tissue-mouse-liver

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Mouse Liver

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - mouse liver tissue

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse liver tissue