CelLytic™ B Cell Lysis Reagent
Protein isolation Bacteria - Pseudomonas aeruginosa
Publication protocol
Overnight cultures of wild-type P. aeruginosa and its derivatives were diluted 100-fold in LB broth and incubated at 37°C until the OD600 reached about 1.5. Total soluble proteins were prepared by using CelLytic B cell lysis reagent (Sigma).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Hollie Fowler
How can I deal with my pellet being too viscous?
I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?
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Manufacturer protocol
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