Protein Expression Eukaryotic cells Hi5

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat mesenchymal stem cells (rMSC)

Get tips on using AllPrep DNA/RNA/Protein Mini Kit to perform Protein isolation Tissue - Human placental tissue

Products Qiagen AllPrep DNA/RNA/Protein Mini Kit

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Products Macherey Nagel NucleoSpin® RNA/Protein

Get tips on using IEF and 2-D Protein Standards to perform Protein Ladder IEF and 2-D Standards

Products Bio-Rad Laboratories IEF and 2-D Protein Standards

Proteins Protein tag Detection of His-tagged proteins

Proteins Protein tag Purification of His-tagged proteins

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human aortic smooth muscle cells (HOSMC)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using Gateway™ pDONR™221 Vector to perform Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5

Products Thermo Fisher Scientific Gateway™ pDONR™221 Vector

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris hmPRα

Products Thermo Fisher Scientific pPICZα A, B, & C Pichia Vectors

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