Site Directed Mutagenesis (SDM) Human Point mutation MDA-MB-231

Get tips on using Human BMP2 ELISA Kit (ab119581) to perform ELISA Human - BMP-2

Get tips on using SLC20A2 Human Gene Knockout Kit to perform CRISPR Human - Repression SLC20A2

Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using IntestiCult™ Organoid Growth Medium (Human) to perform 3D Cell Culture Media Human small intestinal organoids

Get tips on using ON-TARGETplus Human ARL2 (402) siRNA - Individual to perform siRNA / miRNA gene silencing Human - HeLa ARL2

Get tips on using ON-TARGETplus Human MID1 (4281) siRNA - Individual to perform siRNA / miRNA gene silencing Human - LNCap MID1

Get tips on using ON-TARGETplus Human AR (367) siRNA - Individual to perform siRNA / miRNA gene silencing Human - LNCap AR

Get tips on using ON-TARGETplus Human GAPDH (2597) siRNA - Individual to perform siRNA / miRNA gene silencing Human - H1299 GAPDH