siRNA / RNAi /miRNA transfection Human Cells Jurkat cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using SignalSilence® PTEN siRNA to perform siRNA / miRNA gene silencing Human - A2780 PTEN

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using ADAMTS13 siRNA to perform siRNA / miRNA gene silencing Human - HUVEC ADAMTS-13

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LuCaP-77 SKIL

Get tips on using siRNA FOXS1 to perform siRNA / miRNA gene silencing Human - rh-36 FOXS1

Get tips on using siRNA FOXO1 to perform siRNA / miRNA gene silencing Human - CRL-5915 FOXO1

Get tips on using siRNA FOXO1 to perform siRNA / miRNA gene silencing Human - ACC-MESO1 FOXO1