rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Intestine

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized 59M

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized PC-12

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized RIN-5F

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized KG-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.