siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using siRNA(r) NM_053623 Acsl4 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl4

Get tips on using Stealth siRNA(m) Stac3 to perform siRNA / miRNA gene silencing Mouse - C2C12 Stac3

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Pericytes Cx43

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using siGENOME Rat Nrp1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrp1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 PKN3

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Get tips on using Stealth siRNA(r)_Plat to perform siRNA / miRNA gene silencing Rat - NPC tPA/Plat