cell-cycle-assay-human-hela

Get tips on using ProcartaPlex Human Cytokine Panel 1B, 25 plex to perform ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) - -NA- Human -NA-

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MDA-MB-231

Get tips on using Total Exosome RNA & Protein Isolation Kit to perform Protein isolation Mammalian cells - HeLa

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - mouse splenocytes

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Normal human fibroblasts (NHFs)

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Human Tenon fibroblasts