rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using Dynabeads™ mRNA Purification Kit to perform RNA isolation / purification Tissue - Rat Adrenal glands

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Get tips on using Lipofectamine™ 3000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat schwann cells

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Pineal gland

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Lymph node

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Bone marrow