rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using MagExtractor -RNA to perform RNA isolation / purification Cells - immortalized COV-434

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780-CIS

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Salmonella enterica

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Neuro2a