The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion 3T3-L1 TEAD
Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1
Get tips on using pX333-sgNF1-sgTrp53-Cas9 to perform CRISPR Mouse - Deletion 3T3-L1 Trp53
Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Cep350
Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Mouse - Deletion 3T3-L1 MmP13
Get tips on using pSPCas9(BB)-2A-GFP plasmids to perform CRISPR Mouse - Deletion 3T3-L1 Usp2
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment