live-dead-assay-bacteria-corynebacterium-glutamicum

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Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Products Thermo Fisher Scientific LIVE/DEAD Fixable Violet Dead Cell Stain Kit

Get tips on using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays to perform Live / Dead assay bacteria - Staphylococcus aureus

Products Thermo Fisher Scientific LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays

Get tips on using LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays to perform Live / Dead assay bacteria - Borrelia burgdorferi

Products Thermo Fisher Scientific LIVE/DEAD™ BacLight™ Bacterial Viability Kit, for microscopy & quantitative assays

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse keratinocytes

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Products Thermo Fisher Scientific LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

DNA Plasmid Isolation Corynebacterium diphtheriae

Get tips on using LIVE/DEAD™ Yeast Viability Kit to perform Live / Dead assay yeast - Urediniospore

Products Thermo Fisher Scientific LIVE/DEAD™ Yeast Viability Kit

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - H9C2

Products PromoKine Live/Dead cell Staining Kit II

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - HepG2

Products Biovision Live-Dead Cell Staining Kit (BioVision)

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - HUVEC

Products Sigma-Aldrich Live/Dead Cell Double Staining Kit

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