DNA gel extraction / PCR product purification

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Get tips on using Bioanalyzer High Sensitivity DNA Analysis to perform DNA Damage Assay SJSA-1

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)

Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using Apoptotic DNA Ladder Isolation Kit to perform DNA Damage Assay U-87 MG

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.