DNA methylation profiling Gene specific profiling A2780

Get tips on using EpiTect Plus DNA Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Get tips on using Aquadien™ DNA Extraction Kit to perform DNA isolation / purification Bacteria - Gram negative Legionella

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Primary cells Lymphocytes

Get tips on using QIAamp UCP DNA Micro Kit to perform DNA isolation / purification Cells - Immortalized cell lines MKN45

Get tips on using mericon DNA Bacteria Kit (100) to perform DNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)