Microarray RNA amplification

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized T84

Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized JY

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - MIA PaCa-2

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - iPSC-derived cardiomyocytes

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - MDA-MB-231

Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse glial cells

Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Saccharomyces cerevisiae

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using InviTrap Spin Tissue RNA Mini Kit to perform RNA isolation / purification Tissue - Human Artery / Aorta