Get tips on using Purified Mouse Anti-Beclin Clone 20/Beclin (RUO) to perform Autophagy assay cell type - NIH-3T3
Get tips on using MAP LC3α/β Antibody (N-20): sc-16755 to perform Autophagy assay cell type - Gaucher macrophages
Get tips on using Purified Mouse Anti-Beclin Clone 20/Beclin (RUO) to perform Autophagy assay cell type - THP 1
Get tips on using SLC20A2 Human Gene Knockout Kit to perform CRISPR Human - Repression SLC20A2
Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2
Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1
Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74
Get tips on using Brain heart infusion bhi agar 20 plates BD Difco BBL to perform Bacterial cell culture media Bordetella bronchiseptica
Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 ROCK2
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
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