RNA isolation / purification Cells immortalized

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High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Subcutaneous

Products Roche Lifesciences High Pure RNA Isolation Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Skin

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Prostate

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Pancreas

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Nose

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Iris

Products Promega SV Total RNA Isolation System

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Breast

Products Promega SV Total RNA Isolation System

Protein isolation Mammalian cells - Human CD14+ cells Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells Human CD14+ cells

N788 phenol-free total RNA purification kit Product

Get tips on using N788 phenol-free total RNA purification kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Products Amresco N788 phenol-free total RNA purification kit

Direct-zol RNA Kits Product

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - primary human renal proximal tubular epithelial cells

Products Zymo Research Direct-zol RNA Kits

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