rna-isolation-purification-bacteria-gram-negative-bordetella-pertussis

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using Mini Total RNA Kit to perform RNA isolation / purification Cells - immortalized KG-1

Get tips on using Absolutely RNA miRNA Kit to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using ZymoBIOMICS RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized HT-1080

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using E.Z.N.A.® Yeast RNA Kit to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using E.Z.N.A.® Fungal RNA Kit to perform RNA isolation / purification Yeast - Aspergillus nidulans