siRNA / miRNA gene silencing Human Caco-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Caco-2

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis Caco-2

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Ne Caco-2

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - A549 & LTEP-a-2 Lipofectamine

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Caco-2

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - CaCo-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay-Silver to perform DNA Damage Assay Caco-2

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - Caco-2

Get tips on using Monoclonal Anti-ATG5 antibody produced in mouse to perform Autophagy assay cell type - CaCo-2