siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using SLC20A2 Human Gene Knockout Kit to perform CRISPR Human - Repression SLC20A2

Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Human MCP-1 / CCL2 PicoKine™ ELISA Kit to perform ELISA Human - MCP1

Get tips on using Human IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Human - IGF-I

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2

Get tips on using NFE2L2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Get tips on using Kdm2b siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 Fbxl10