Protein expression and purification Mammalian cells HEK 293

Get tips on using pANC231 (xylE) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylE

Get tips on using pANC230 (xylD) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylD

Get tips on using pANC223 (xylC) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylC

Get tips on using pANC210 (xylB) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylB

Get tips on using pANC209 (xylA) to perform Protein Expression Prokaryotic cells - A. cellulolyticus xylA

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human CD14+ cells

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I

Get tips on using pZH713 to perform Protein Expression Prokaryotic cells - E. coli PP7cp-SYFP2