Cell line authentication

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay A-375

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HCT 116

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay DLD-1

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Caco-2

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Hep G2

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Capan-2

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Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay SJSA-1

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HEK 293T

Products Bio-Techne CometAssay Single Cell Gel Electrophoresis Assay

Get tips on using Cell Cycle Assay Kit (Fluorometric - Green) to perform DNA quantification Human - Hep G2

Products Abcam Cell Cycle Assay Kit (Fluorometric - Green)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type K562 cells

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