siRNA / miRNA gene silencing Human A172

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299