Microarray Gene expression arrays Rat pancreas tissue

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Colon

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Adipose

Get tips on using BioMag Goat Anti-Rat IgG (500 ml) to perform Cell Isolation Mouse T cells

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Tissue - Rat Ovaries

Get tips on using RNAprotect Tissue Reagent (250 ml) to perform RNA stabilization Sorted cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.