Protein expression and purification Bacteria DH10Bac™

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Get tips on using pLsLF-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - N. benthamiana proinsulin

Get tips on using pLDutr-CTB-Fx3Pris to perform Protein Expression Eukaryotic cells - lettuce leaves proinsulin

Get tips on using pKS2-ST::CBHII to perform Protein Expression Eukaryotic cells - W. anomalus CBHII

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T

Get tips on using RNAGEM™ Tissue PLUS to perform RNA isolation / purification Tissue - Rat Pineal gland

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized K-562

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Cells - immortalized MA-104