angiogenesis-assay-human-huvec

Get tips on using Human LBP ELISA Kit (ab213805) to perform ELISA Human - LBP

Get tips on using Human HSP70/HSPA1A DuoSet ELISA to perform ELISA Human - HSP70

Get tips on using Human Fibronectin ELISA Kit (ab108847) to perform ELISA Human - Fibronectin

Get tips on using I-FABP, Human, ELISA kit to perform ELISA Human - FABP2

Get tips on using Human BDNF ELISA Kit (ab99978) to perform ELISA Human - BDNF

Get tips on using Human/Mouse BDNF DuoSet ELISA to perform ELISA Human - BDNF

Get tips on using Human Adiponectin ELISA Kit (ab108786) to perform ELISA Human - Adiponectin

Get tips on using Human Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Human - Adiponectin

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.