shRNA gene silencing Human HEK 293T CAPN5- (Calpains)

- Found 5600 results

Get tips on using Mouse Gene Expression v2 4x44K Microarray Kit to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Products Agilent Technologies Mouse Gene Expression v2 4x44K Microarray Kit

Get tips on using Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - Hep3B

Products Thermo Fisher Scientific Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Products Thermo Fisher Scientific TaqMan® MicroRNA Reverse Transcription Kit

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Rat pancreas tissue Cyanine 3 & cyanine 5

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates U87 and U251 glioblastoma cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates rat mesenchymal stem cells (MSCs)

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Hep3B SFRP3

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Hep3B SPRY1

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling HepG2 FHIT

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling HepG2 Fibrinogen

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms