siRNA / miRNA gene silencing Human Caco-2

Get tips on using BspPI (AlwI) (2 U/µL) to perform Restriction Enzymes AlwI / BspPI

Get tips on using TIMP-2 Antibody (B-12): sc-365671 to perform Western blotting TIMP-2

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using Rat BMP-2 PicoKine™ ELISA Kit to perform ELISA Rat - BMP-2

Get tips on using MAP LC3β Antibody (G-2) to perform Autophagy assay cell type - AR42J

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using BLOOD AGAR BASE NO.2 to perform Bacterial cell culture media Helicobacter pylori

Get tips on using Human BMP2 ELISA Kit (ab119581) to perform ELISA Human - BMP-2

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.