Wound healing assay cell type rat

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - A549

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - HepG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - K562

Get tips on using Autophagy/Cytotoxicity Dual Staining Kit to perform Autophagy assay cell type - Macrophages

Get tips on using Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) to perform siRNA / miRNA gene silencing Rat - NRCM Flot1

Get tips on using Flot2 Rat siRNA Oligo Duplex (Locus ID 83764) to perform siRNA / miRNA gene silencing Rat - NRCM Flot2

Get tips on using ANGPTL3 (mouse/rat) Dual ELISA Kit to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

Get tips on using Orai1 Rat siRNA Oligo Duplex (Locus ID 304496) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Orai1

Get tips on using LAMP-1 Antibody (H4A3) to perform Autophagy assay cell type - Proximal tubular cells (rPT)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.