siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation A549 ISG15

Get tips on using ON-TARGETplus Rat Hspa5 (25617) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - AR42J Grp78/Hspa5

Get tips on using Stealth siRNA(r)_Mmp15 to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) mmp15

Get tips on using Stealth siRNA(r)_Ctnnb1 to perform siRNA / miRNA gene silencing Rat - NPC β-Catenin/Ctnnb1

Get tips on using SMARTpool: siGENOME Fancd2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16 Melanoma cells FANCD2

Get tips on using siGENOME Mouse Amotl2 (56332) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 AmotL2

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using siGENOME Rat Gata1 (25172) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) Gata1

Get tips on using siGENOME Mouse Alox12 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 12-Lox/ALOX12

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.