Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - U266
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - Jurkat
Get tips on using PI/RNASE Solution to perform Cell cycle assay human - K562
DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.
Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse MS1
Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse 3T3-L1
Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - HT-29
Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MCF-7
Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - SH-SY5Y
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