DNA methylation profiling Gene specific profiling Ca Ski

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Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - SH-SY5Y

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I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

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