RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Skin

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Prostate

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Pancreas

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Nose

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Iris

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Breast

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

Get tips on using N788 phenol-free total RNA purification kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.