siRNA / RNAi /miRNA transfection Rat Ar4-2j (Pancreatic tumor)

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) BCPAP

Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34

Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Primary cells Rat schwann cells

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Get tips on using Rat VEGF PicoKine™ ELISA Kit to perform ELISA Rat - VEGF