DNA methylation profiling Gene specific profiling Ca Ski

Get tips on using EZ-10 Spin Column Plasmid DNA Miniprep Kit to perform Plasmid Isolation Cronobacter sakazakii

Get tips on using Easy-Spin (DNA free) Total RNA Extraction Kit to perform RNA isolation / purification Tissue - Mouse Cornea

Get tips on using Wizard® Plus Maxipreps DNA Purification System to perform Plasmid Isolation E. coli DH5α

Get tips on using EZ-10 Spin Column Plasmid DNA Miniprep Kit to perform Plasmid Isolation E. coli-S. cerevisiae transconjugate

Get tips on using Easy-Spin (DNA free) Total RNA Extraction Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - yeast, Yarrowia lipolytica

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.