DNA isolation / purification Tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Intestine

Get tips on using RNeasy MinElute Cleanup Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Joints

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Jejunum

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - Human Gut

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Esophagus

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.