angiogenesis-assay-human-huvec

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using psiCHECK-2vector to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Get tips on using CometChip Electrophoresis Starter Kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using Fluorometric Intracellular Ros Kit to perform ROS assay cell type - SH-SY5Y human neuroblastoma

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma

Get tips on using Cell Death Detection ELISA to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using In situ apoptosis detection to perform Apoptosis assay cell type - Human endometrial stromal cells

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

Get tips on using ON-TARGETplus Human PPARGC1B siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PGC-1β/PPARGC1B