dna-methylation-profiling-gene-specific-profiling-siha-hpv-16

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DNA DNA isolation / purification Tissue bronchoalveolar lavage

DNA DNA isolation / purification Plants Mycelial mats

DNA PCR Conventional / Qualitative PCR Viral DNA

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using Wizard® Plus Maxipreps DNA Purification System to perform Plasmid Isolation E. coli DH5α

Products Promega Wizard® Plus Maxipreps DNA Purification System

Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Fisher Scientific Illustra GFX PCR DNA and Gel Band Purification kit

Get tips on using ARCTURUS® PicoPure® DNA Extraction Kit to perform RNA isolation / purification Cells - primary human melanocytes

Products Thermo Fisher Scientific ARCTURUS® PicoPure® DNA Extraction Kit

Get tips on using Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - yeast, Yarrowia lipolytica

Products Thermo Fisher Scientific Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

RNA siRNA / miRNA gene silencing Human Jurkat GAPDH Lipid

RNA siRNA / miRNA gene silencing Human HepG2 AMPKα1/α2

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