RNA isolation / purification Tissue Human

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Human blood cord

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using MagCellect Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using B Cell Isolation Kit II, human to perform Cell Isolation B cell

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mononuclear cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts