siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - HeLa Cdc20

Get tips on using SASI_Hs01_00024301 to perform siRNA / miRNA gene silencing Human - MOLT4 RAG1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - LLC PKN3

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - HeLa PKN3

Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX

Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1

Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL

Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)