siRNA / miRNA gene silencing Human THP-1

Get tips on using HO-1 (rat), ELISA kit to perform ELISA Rat - HO-1

Get tips on using Endothelin-1 Quantikine ELISA Kit to perform ELISA Rat - Endothelin 1

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Rat - Endothelin 1

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Deletion 3T3-L1 Epac1

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

Get tips on using pGEX-4T-1 to perform Protein Expression Prokaryotic cells - E. coli LsrK

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Get tips on using mTeSR™1 to perform Stem cell culture media hESC lines H9, H1

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD133